An alternative beads-on-a-string chromatin architecture in Thermococcus kodakarensis.
EMBO Rep
; 14(8): 711-7, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23835508
ABSTRACT
We have applied chromatin sequencing technology to the euryarchaeon Thermococcus kodakarensis, which is known to possess histone-like proteins. We detect positioned chromatin particles of variable sizes associated with lengths of DNA differing as multiples of 30 bp (ranging from 30 bp to >450 bp) consistent with formation from dynamic polymers of the archaeal histone dimer. T. kodakarensis chromatin particles have distinctive underlying DNA sequence suggesting a genomic particle-positioning code and are excluded from gene-regulatory DNA suggesting a functional organization. Beads-on-a-string chromatin is therefore conserved between eukaryotes and archaea but can derive from deployment of histone-fold proteins in a variety of multimeric forms.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Histonas
/
Nucleossomos
/
Thermococcus
/
Proteínas Arqueais
/
DNA Arqueal
/
Genoma Arqueal
Idioma:
En
Revista:
EMBO Rep
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Japão