Dual-aptamer-based biosensing of toxoplasma antibody.
Anal Chem
; 85(17): 8354-60, 2013 Sep 03.
Article
em En
| MEDLINE
| ID: mdl-23899133
ABSTRACT
A panel of seven aptamers to antitoxoplasma IgG is first discovered in this report. The aptamers are selected using systematic evolution of ligands by exponential enrichment (SELEX) technology, cloned, and identified by sequencing and affinity assay. Among them, two aptamers (TGA6 and TGA7) with the highest affinities are employed as capture probe and detection probe in developing a quantum dots-labeled dual aptasensor (Q-DAS). In the presence of antitoxoplasma IgG, an aptamer-protein-aptamer sandwich complex (TGA6-IgG-TGA7) is formed and captured on a multiwell microplate, whose fluorescence can be read out using quantum dots as the fluorescence label, ensuring highly sensitive and specific sensing of antitoxoplasma IgG. The operating characteristics of the proposed assay are guaranteed using dual aptamers as the recognizing probes when compared with antibody-based immunoassay. Q-DAS has a linearity within the range of 0.5-500 IU with a lowest detection of 0.1 IU. Receiver operating curves of 212 clinical samples show a 94.8% sensitivity and 95.7% specificity when the cutoff value is set as 6.5 IU, indicating the proposed Q-DAS is a promising assay in large-scale screening of toxoplasmosis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Toxoplasma
/
Anticorpos Antiprotozoários
/
Técnicas Biossensoriais
/
Aptâmeros de Nucleotídeos
/
Técnica de Seleção de Aptâmeros
Tipo de estudo:
Prognostic_studies
Limite:
Adult
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China