Rapid, isothermal DNA self-replication induced by a destabilizing lesion.
Angew Chem Int Ed Engl
; 52(40): 10577-81, 2013 Sep 27.
Article
em En
| MEDLINE
| ID: mdl-23922255
ABSTRACT
You spin me round Using a destabilizing abasic site and high concentration of ligase, rapid DNA self-replication in an isothermal ligase chain reaction (LCR) was produced. Both destabilization and rapid ligation are essential for proper LCR replication. This method also provides insight into prebiotic nucleotide replication and is a potential amplification method for biodiagnostics.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
DNA
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Técnicas de Amplificação de Ácido Nucleico
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Reação em Cadeia da Ligase
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Replicação do DNA
Limite:
Humans
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Ano de publicação:
2013
Tipo de documento:
Article