Increase in stearidonic acid by increasing the supply of histidine to oleaginous Saccharomyces cerevisiae.

Kimura, Kazuyoshi; Kamisaka, Yasushi; Uemura, Hiroshi; Yamaoka, Masakazu

Kimura, Kazuyoshi; Kamisaka, Yasushi; Uemura, Hiroshi; Yamaoka, Masakazu.

Afiliação

Kimura K; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan. Electronic address: kykimura@ni.aist.go.jp.
Kamisaka Y; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.
Uemura H; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.
Yamaoka M; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.

J Biosci Bioeng ; 117(1): 53-6, 2014 Jan.

Article em En | MEDLINE | ID: mdl-23932357

ABSTRACT

ABSTRACT

Increasing concentration of histidine significantly increased stearidonic acid production and cell growth in oleaginous Saccharomyces cerevisiae that has been genetically modified by Δsnf2 disruption, DGA1 and Δ6 desaturase gene overexpression, and LEU2 expression. High concentration of histidine in wild-type transformant and HIS3 expression in Δsnf2 transformant also increased stearidonic acid.

Assuntos

Ácidos Graxos Ômega-3/metabolismo; Histidina/metabolismo; Saccharomyces cerevisiae/metabolismo; 3-Isopropilmalato Desidrogenase/genética; 3-Isopropilmalato Desidrogenase/metabolismo; Adenosina Trifosfatases/genética; Adenosina Trifosfatases/metabolismo; Diacilglicerol O-Aciltransferase/genética; Diacilglicerol O-Aciltransferase/metabolismo; Saccharomyces cerevisiae/genética; Proteínas de Saccharomyces cerevisiae/genética; Proteínas de Saccharomyces cerevisiae/metabolismo; Fatores de Transcrição/genética; Fatores de Transcrição/metabolismo

Palavras-chave

DGA1; Histidine; Polyunsaturated fatty acid; Stearidonic acid; Δ6 Desaturase; Δsnf2; α-Linolenic acid

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Ácidos Graxos Ômega-3 / Histidina Idioma: En Revista: J Biosci Bioeng Assunto da revista: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Ano de publicação: 2014 Tipo de documento: Article

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