Posttranscriptional self-regulation by the Lyme disease bacterium's BpuR DNA/RNA-binding protein.
J Bacteriol
; 195(21): 4915-23, 2013 Nov.
Article
em En
| MEDLINE
| ID: mdl-23974034
ABSTRACT
Bacteria require explicit control over their proteomes in order to compete and survive in dynamic environments. The Lyme disease spirochete Borrelia burgdorferi undergoes substantial protein profile changes during its cycling between vector ticks and vertebrate hosts. In an effort to understand regulation of these transitions, we recently isolated and functionally characterized the borrelial nucleic acid-binding protein BpuR, a PUR domain-containing protein. We now report that this regulatory protein governs its own synthesis through direct interactions with bpuR mRNA. In vitro and in vivo techniques indicate that BpuR binds with high affinity and specificity to the 5' region of its message, thereby inhibiting translation. This negative feedback could permit the bacteria to fine-tune cellular BpuR concentrations. These data add to the understanding of this newly described class of prokaryotic DNA- and RNA-binding regulatory proteins.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doença de Lyme
/
Regulação Bacteriana da Expressão Gênica
/
Processamento Pós-Transcricional do RNA
/
Proteínas de Ligação a RNA
/
Borrelia burgdorferi
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos