Direct and selective tagging of cysteine residues in peptides and proteins with 4-nitropyridyl lanthanide complexes.
Chem Commun (Camb)
; 49(80): 9104-6, 2013 Oct 14.
Article
em En
| MEDLINE
| ID: mdl-23989726
ABSTRACT
A cysteine-selective tagging method in water is reported, based on rapid displacement of a pyridyl nitro-substituent in simple pyridines and lanthanide complexes. The conjugation reaction creates a short link between the tag and peptide, holding the peptide closer to the Ln(3+) ion and with reduced flexibility compared to existing methods.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Piridinas
/
Proteínas
/
Cisteína
/
Elementos da Série dos Lantanídeos
/
Complexos de Coordenação
Idioma:
En
Revista:
Chem Commun (Camb)
Assunto da revista:
QUIMICA
Ano de publicação:
2013
Tipo de documento:
Article