[Study on effect of weinaokang and bilobalide on autophagy and neurogenesis induced by focal cerebral ischemia reperfusion].
Zhongguo Zhong Yao Za Zhi
; 38(13): 2182-6, 2013 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-24079250
ABSTRACT
OBJECTIVE:
To observe the protective effect of the Weinaokang (WNK) and its active compound bilobalide on focal cerebral ischemia reperfusion, and their mechanisms.METHOD:
The 60-minute middle cerebral artery occlusion (MCAO) was adopted to establish the 24 h-14 d reperfusion model. The expression of Beclin-1 was detected by the Western blotting technique. The transmission electron microscopy was used to observe ultrastructural changes. Neurogenesis was detected by the immunofluorescence staining.RESULT:
WNK (20, 10 mg x kg(-1), ig) or its active compound bilobalide (10, 5 mg x kg(-1), ig) could promote the generation of mature neurons (BrdU(+) -MAP-2+) at the ischemic side, and inhibit expression of autophagy-related gene Beclin-1, so as to reduce the neuron injury induced by focal cerebral ischemia reperfusion.CONCLUSION:
WNK and its active compound bilobalide can inhibit neuron autophagy and improve neurogenesis in ischemic peripheral area, suggesting that neurogenesis may be the intervention target for WNK to promote self-repairing of ischemic area.
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Base de dados:
MEDLINE
Assunto principal:
Autofagia
/
Medicamentos de Ervas Chinesas
/
Traumatismo por Reperfusão
/
Isquemia Encefálica
/
Ciclopentanos
/
Ginkgolídeos
/
Neurogênese
/
Furanos
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Assunto da revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China