Cross-serotype immunity induced by immunization with a conserved rhinovirus capsid protein.
PLoS Pathog
; 9(9): e1003669, 2013.
Article
em En
| MEDLINE
| ID: mdl-24086140
ABSTRACT
Human rhinovirus (RV) infections are the principle cause of common colds and precipitate asthma and COPD exacerbations. There is currently no RV vaccine, largely due to the existence of â¼150 strains. We aimed to define highly conserved areas of the RV proteome and test their usefulness as candidate antigens for a broadly cross-reactive vaccine, using a mouse infection model. Regions of the VP0 (VP4+VP2) capsid protein were identified as having high homology across RVs. Immunization with a recombinant VP0 combined with a Th1 promoting adjuvant induced systemic, antigen specific, cross-serotype, cellular and humoral immune responses. Similar cross-reactive responses were observed in the lungs of immunized mice after infection with heterologous RV strains. Immunization enhanced the generation of heterosubtypic neutralizing antibodies and lung memory T cells, and caused more rapid virus clearance. Conserved domains of the RV capsid therefore induce cross-reactive immune responses and represent candidates for a subunit RV vaccine.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Rhinovirus
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Proteínas do Capsídeo
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Anticorpos Neutralizantes
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Pulmão
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Anticorpos Antivirais
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
PLoS Pathog
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Reino Unido