Nanoscale ligand spacing influences receptor triggering in T cells and NK cells.
Nano Lett
; 13(11): 5608-14, 2013.
Article
em En
| MEDLINE
| ID: mdl-24125583
ABSTRACT
Bioactive nanoscale arrays were constructed to ligate activating cell surface receptors on T cells (the CD3 component of the TCR complex) and natural killer (NK) cells (CD16). These arrays are formed from biofunctionalized gold nanospheres with controlled interparticle spacing in the range 25-104 nm. Responses to these nanoarrays were assessed using the extent of membrane-localized phosphotyrosine in T cells stimulated with CD3-binding nanoarrays and the size of cell contact area for NK cells stimulated with CD16-binding nanoarrays. In both cases, the strength of response decreased with increasing spacing, falling to background levels by 69 nm in the T cell/anti-CD3 system and 104 nm for the NK cell/anti-CD16 system. These results demonstrate that immune receptor triggering can be influenced by the nanoscale spatial organization of receptor/ligand interactions.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Complexo Receptor-CD3 de Antígeno de Linfócitos T
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Nanotecnologia
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Nanopartículas
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Receptores de Células Matadoras Naturais
Limite:
Humans
Idioma:
En
Revista:
Nano Lett
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Reino Unido