[Detection of carbapenem-producing Enterobacteriaceae by MALDI-TOF MS].
Zhonghua Yi Xue Za Zhi
; 93(26): 2079-81, 2013 Jul 09.
Article
em Zh
| MEDLINE
| ID: mdl-24169292
ABSTRACT
OBJECTIVE:
To evaluate a rapid matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) assay in detecting carbapenemase-producing Enterobacteriaceae.METHODS:
Thirty-four carbapenemase-producing Enterobacteriaceae and 50 carbapenem susceptible Enterobacteriaceae clinical isolates were tested. After a 2-hour incubation of bacteria with meropenem, the mixtures were centrifuged and the supernatants analyzed by MALDI-TOF MS.RESULTS:
All spectra of carbapenem susceptible Enterobacteriaceae showed peaks of m/z 384, 406, 428 while those of carbapenemase-producing Enterobacteriaceae m/z 358, 380, 402, 424, 446, 468. And the assay of MALDI-TOF MS was highly consistent with sequencing (P = 1.000).CONCLUSION:
MALDI-TOF MS can detect carbapenemase-producing Enterobacteriaceae rapidly and accurately.
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Enterobacteriaceae
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Zhonghua Yi Xue Za Zhi
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China