Preparation of cells for microscopy using chamber slides and coverslips.
Methods Enzymol
; 533: 241-7, 2013.
Article
em En
| MEDLINE
| ID: mdl-24182929
ABSTRACT
Microscopy is a simple, direct technique for examining the morphology of cells and their organelles. Cells are immobilized on a solid support that is optically suitable for microscopy, and then fixed. When coupled with antibody-based immunofluorescent or immunocytochemical methods, the expression of specific proteins can be quantified and localized. Specialized stains and dyes can also be used to visualize nucleic acids or other cellular structures. See alternative protocols for fixation of suspension cells on Preparation of Cells for Microscopy using Cytospin and Preparation of Cells for Microscopy using 'Cell Blocks'.
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MEDLINE
Assunto principal:
Técnicas Citológicas
/
Microscopia
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2013
Tipo de documento:
Article