A label-free microfluidic biosensor for activity detection of single microalgae cells based on chlorophyll fluorescence.
Sensors (Basel)
; 13(12): 16075-89, 2013 Nov 26.
Article
em En
| MEDLINE
| ID: mdl-24287532
Detection of living microalgae cells is very important for ballast water treatment and analysis. Chlorophyll fluorescence is an indicator of photosynthetic activity and hence the living status of plant cells. In this paper, we developed a novel microfluidic biosensor system that can quickly and accurately detect the viability of single microalgae cells based on chlorophyll fluorescence. The system is composed of a laser diode as an excitation light source, a photodiode detector, a signal analysis circuit, and a microfluidic chip as a microalgae cell transportation platform. To demonstrate the utility of this system, six different living and dead algae samples (Karenia mikimotoi Hansen, Chlorella vulgaris, Nitzschia closterium, Platymonas subcordiformis, Pyramidomonas delicatula and Dunaliella salina) were tested. The developed biosensor can distinguish clearly between the living microalgae cells and the dead microalgae cells. The smallest microalgae cells that can be detected by using this biosensor are 3 µm ones. Even smaller microalgae cells could be detected by increasing the excitation light power. The developed microfluidic biosensor has great potential for in situ ballast water analysis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Poluentes Químicos da Água
/
Técnicas Biossensoriais
/
Clorofila
/
Microfluídica
/
Microalgas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Sensors (Basel)
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China