An efficient and economical method for extraction of DNA amenable to biotechnological manipulations, from diverse soils and sediments.
J Appl Microbiol
; 116(4): 923-33, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24329912
ABSTRACT
AIMS:
An attempt was made to optimize a new protocol for isolation of pure metagenomic DNA from soil samples. METHODS ANDRESULTS:
Various chemicals (FeCl3 , MgCl2 , CaCl2 and activated charcoal) were tested for their efficacy in isolation of metagenomic DNA from different soil and compost samples. Among these trials, charcoal and MgCl2 when used in combination yielded highly pure DNA free from humic acids and other contaminants. The DNA extracted with the optimized protocol was readily digested, amplified and cloned. Moreover, compared with a well-established commercial DNA isolation kit (UltraClean™ Soil DNA Isolation Kit), our method for DNA isolation was found to be economical. This demonstrated that the method developed can be applied to a wide variety of soil samples and allows handling of multiple samples at a given time.CONCLUSIONS:
The optimized protocol developed has successfully yielded pure metagenomic DNA amenable to biotechnological manipulations. SIGNIFICANCE AND IMPACT OF THE STUDY A user-friendly and economical protocol for isolation of DNA from soil and compost samples has been developed.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Microbiologia do Solo
/
DNA Bacteriano
/
DNA Fúngico
/
Metagenômica
Tipo de estudo:
Health_economic_evaluation
Idioma:
En
Revista:
J Appl Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Índia