A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies.

Wilkinson, Robert N; Elworthy, Stone; Ingham, Philip W; van Eeden, Fredericus J M

Wilkinson, Robert N; Elworthy, Stone; Ingham, Philip W; van Eeden, Fredericus J M.

Afiliação

Wilkinson RN; Department of Cardiovascular Science, University of Sheffield, Medical School, Sheffield, United Kingdom.
Elworthy S; Department of Biomedical Science/ MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield, United Kingdom.
Ingham PW; Department of Biomedical Science/ MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield, United Kingdom.
van Eeden FJ; Department of Biomedical Science/ MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield, United Kingdom.

Biotechniques ; 55(6): 314-6, 2013 Dec.

Article em En | MEDLINE | ID: mdl-24344681

ABSTRACT

ABSTRACT

Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.

Assuntos

Técnicas de Genotipagem; Peixe-Zebra/genética; Animais; Biópsia/veterinária; Sequenciamento de Nucleotídeos em Larga Escala; Larva/genética; Reação em Cadeia da Polimerase/métodos; Cauda/patologia; Peixe-Zebra/crescimento & desenvolvimento

Palavras-chave

PCR; embryo; fin clip; genotyping; zebrafish

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peixe-Zebra / Técnicas de Genotipagem Limite: Animals Idioma: En Revista: Biotechniques Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Reino Unido

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