The effect of vascular endothelial growth factor overexpression in experimental necrotizing enterocolitis.
Pediatr Surg Int
; 30(3): 327-32, 2014 Mar.
Article
em En
| MEDLINE
| ID: mdl-24380924
ABSTRACT
PURPOSE:
Necrotizing enterocolitis (NEC) is a serious condition, predominantly observed in premature infants. We used an experimental NEC model to investigate the effects of vascular endothelial growth factor (VEGF) cloned into a plasmid. MATERIALS ANDMETHODS:
Twenty-four newborn Wistar albino rats were randomized equally into three groups as follows control, NEC and NEC+VEGF. NEC was induced by hyperosmolar enteral formula feeding, exposure to hypoxia/reoxygenation and cold stress. In the NEC+VEGF group, VEGF (1 µg) incorporated into plasmid (2 µg) was administered subcutaneously once daily for a total of 3 days starting on the first day of the NEC procedure. All rats were sacrificed on the 4th day of life, and the specimens were harvested for histopathological and biochemical examinations [including tissue oxidative stress (malondialdehyde and nitric oxide), inflammation (myeloperoxidase, interleukin-6 and tumor necrosis factor alpha) and apoptosis (caspase-3 activity) parameters].RESULTS:
In the NEC+VEGF group, tissue malondialdehyde, nitric oxide, interleukin-6, tumor necrosis factor alpha levels and caspase-3 activity were significantly decreased. In addition, the myeloperoxidase level was increased compared to that of the NEC group (p < 0.05). Histopathologically, VEGF overexpression enhanced angiogenesis, alleviated villous atrophy and tissue edema (p < 0.05).CONCLUSION:
VEGF overexpression with plasmids seems to be a promising approach in the management of NEC.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Enterocolite Necrosante
/
Fator A de Crescimento do Endotélio Vascular
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Pediatr Surg Int
Assunto da revista:
PEDIATRIA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Turquia