Base specificity in the interaction of ethidium with synthetic polyribonucleotides.
Nucleic Acids Res
; 15(14): 5803-12, 1987 Jul 24.
Article
em En
| MEDLINE
| ID: mdl-2441360
ABSTRACT
Base specificity in the interaction of ethidium with double stranded synthetic RNA homopolymers has been studied by means of spectroscopic (UV-visible absorption and fluorescence), microcalorimetric and dilatometric techniques. The results show a strong base specificity in this interaction, the association constant with poly Apoly U being more than three order of magnitude higher than with poly Opoly C. The interaction is mainly enthalpy driven, the differences in affinity being essentially entropic in origin. These evidences along with the dilatometric data suggest that the observed base specificity may arise from the different extent of water release upon intercalation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polirribonucleotídeos
/
Etídio
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
1987
Tipo de documento:
Article