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A dynamin homolog promotes the transition from hemifusion to content mixing in intracellular membrane fusion.
Kulkarni, Aditya; Alpadi, Kannan; Sirupangi, Tirupataiah; Peters, Christopher.
Afiliação
  • Kulkarni A; Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
Traffic ; 15(5): 558-71, 2014 May.
Article em En | MEDLINE | ID: mdl-24471450
The convergence of the antagonistic reactions of membrane fusion and fission at the hemifusion/hemifission intermediate has generated a captivating enigma of whether Soluble N-ethylmaleimide sensitive factor Attachment Protein Receptor (SNAREs) and dynamin have unusual counter-functions in fission and fusion, respectively. SNARE-mediated fusion and dynamin-driven fission are fundamental membrane flux reactions known to occur during ubiquitous cellular communication events such as exocytosis, endocytosis and vesicle transport. Here we demonstrate the influence of the dynamin homolog Vps1 (Vacuolar protein sorting 1) on lipid mixing and content mixing properties of yeast vacuoles, and on the incorporation of SNAREs into fusogenic complexes. We propose a novel concept that Vps1, through its oligomerization and SNARE domain binding, promotes the hemifusion-content mixing transition in yeast vacuole fusion by increasing the number of trans-SNAREs.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dinaminas / Membranas Intracelulares / Fusão de Membrana Idioma: En Revista: Traffic Assunto da revista: FISIOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dinaminas / Membranas Intracelulares / Fusão de Membrana Idioma: En Revista: Traffic Assunto da revista: FISIOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos