Genome-wide miRNA-profiling of aflatoxin B1-induced hepatic injury using deep sequencing.
Toxicol Lett
; 226(2): 140-9, 2014 Apr 21.
Article
em En
| MEDLINE
| ID: mdl-24472605
ABSTRACT
Aflatoxin B1 is a potent carcinogen which can induce** hepatocellular carcinoma (HCC) in mammals. Though microRNAs are known to play important roles in tumorigenesis, the functional complexity of microRNAs in AFB1-induced hepatocellular tumorigenesis has not yet been elucidated. Here, we applied Illumina deep sequencing technology for high-throughput profiling of microRNA in rat liver tissue before and after treatment with aflatoxin B1. Analysis of mature miRNAs from different arms of pre-miRNAs allowed us to identify the predominant form of miRNA. We studied the differential expression profile of miRNAs in two libraries, identifying several cancer-related microRNAs which exhibit abnormal expression. KEGG analysis indicated that predicted target genes of differentially expressed miRNAs are involved in cancer-related pathways. Bioinformatic analysis predicted 16 potential novel miRNAs. Our work provides new insights at the miRNA level into AFB1-induced hepatic injury which may lead to HCC.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Precursores de RNA
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Aflatoxina B1
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Perfilação da Expressão Gênica
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MicroRNAs
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Doença Hepática Induzida por Substâncias e Drogas
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Sequenciamento de Nucleotídeos em Larga Escala
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Fígado
Tipo de estudo:
Etiology_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Toxicol Lett
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China