HLA-G1 increases the radiosensitivity of human tumoral cells.
Cell Immunol
; 287(2): 106-11, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24487034
ABSTRACT
Different molecules regulate the response of tumoral tissues to ionizing radiation. The objective of this work was to determine if HLA-G1 expression modulates the radiosensitivity of human tumoral cell lines. To this end, human melanoma M8 and human erythroleukemia K562 cell lines, with their correspondent HLA-G1 negative and positive variants, were gamma irradiated and the survival frequency was determined by clonogenic assay. The survival fraction of HLA-G1 expressing cells was around 60% of HLA-G1 negative cells. The generation of acidic vesicular organelles was higher in HLA-G1 positive cells. Apoptosis levels showed statistically significant differences only in K562 cells, whereas the variation in G2/M cycle progression was only significant in M8 cells. In addition, irradiation diminished cell-surface HLA-G1 and increased soluble HLA-G1 levels. Soluble HLA-G1 has no influence on cell survival in any cell line. In summary, we could demonstrate that HLA-G1 confers higher radiosensitivity to HLA-G1 expressing cells.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Tolerância a Radiação
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Biomarcadores Tumorais
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Leucemia Eritroblástica Aguda
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Antígenos HLA-G
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Melanoma
Limite:
Humans
Idioma:
En
Revista:
Cell Immunol
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Argentina