Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector.
Croat Med J
; 55(1): 45-9, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24577826
ABSTRACT
AIM:
To investigate the survival of laboratory rats after irradiation and to study the cellularity of their bone marrow and the multipotential mesenchymal stem cells (BM-MSCs) in groups treated with or without a new thiol-based radioprotector (GM2011).METHODS:
Animals were irradiated by a Cobalt gamma source at 6.7 Gy. Treated animals were given i.p. GM2011 30 minutes before and 3 and 7 hours after irradiation. Controls consisted of sham irradiated animals without treatment and animals treated without irradiation. After 30 days post-irradiation, animals were sacrificed and bone marrow cells were prepared from isolated femurs. A colony forming unit-fibroblast (CFU-F) assay was performed to obtain the number of BM-MSCs.RESULTS:
In the treated group, 87% of animals survived, compared to only 30% in the non-treated irradiated group. Irradiation induced significant changes in the bone marrow of the treated rats (total bone marrow cellularity was reduced by~60%--from 63 to 28 cells × 10(6)/femur and the frequency of the CFU-F per femur by~70% - from 357 to 97), however GL2011 almost completely prevented the suppressive effect observed on day 30 post-irradiation (71 cells × 10(6)/femur and 230 CFU-F/femur).CONCLUSION:
Although the irradiation dosage was relatively high, GL2011 acted as a very effective new radioprotector. The recovery of the BN-MSCs and their counts support the effectiveness of the studied radioprotector.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Lesões Experimentais por Radiação
/
Protetores contra Radiação
/
Regeneração
/
Medula Óssea
/
Células-Tronco Mesenquimais
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Croat Med J
Assunto da revista:
MEDICINA
Ano de publicação:
2014
Tipo de documento:
Article