Impact of inhaled nitric oxide on the sulfatide profile of neonatal rat brain studied by TOF-SIMS imaging.
Int J Mol Sci
; 15(4): 5233-45, 2014 Mar 25.
Article
em En
| MEDLINE
| ID: mdl-24670476
ABSTRACT
Despite advances in neonatal intensive care leading to an increased survival rate in preterm infants, brain lesions and subsequent neurological handicaps following preterm birth remain a critical issue. To prevent brain injury and/or enhance repair, one of the most promising therapies investigated in preclinical models is inhaled nitric oxide (iNO). We have assessed the effect of this therapy on brain lipid content in air- and iNO-exposed rat pups by mass spectrometry imaging using a time-of-flight secondary ion mass spectrometry (TOF-SIMS) method. This technique was used to map the variations in lipid composition of the rat brain and, particularly, of the white matter. Triplicate analysis showed a significant increase of sulfatides (25%-50%) in the white matter on Day 10 of life in iNO-exposed animals from Day 0-7 of life. These robust, repeatable and semi-quantitative data demonstrate a potent effect of iNO at the molecular level.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sulfoglicoesfingolipídeos
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Lesões Encefálicas
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Fármacos Neuroprotetores
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Substância Branca
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Óxido Nítrico
Limite:
Animals
Idioma:
En
Revista:
Int J Mol Sci
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
França