Quality metrics for stem cell-derived cardiac myocytes.
Stem Cell Reports
; 2(3): 282-94, 2014 Mar 11.
Article
em En
| MEDLINE
| ID: mdl-24672752
ABSTRACT
Advances in stem cell manufacturing methods have made it possible to produce stem cell-derived cardiac myocytes at industrial scales for in vitro muscle physiology research purposes. Although FDA-mandated quality assurance metrics address safety issues in the manufacture of stem cell-based products, no standardized guidelines currently exist for the evaluation of stem cell-derived myocyte functionality. As a result, it is unclear whether the various stem cell-derived myocyte cell lines on the market perform similarly, or whether any of them accurately recapitulate the characteristics of native cardiac myocytes. We propose a multiparametric quality assessment rubric in which genetic, structural, electrophysiological, and contractile measurements are coupled with comparison against values for these measurements that are representative of the ventricular myocyte phenotype. We demonstrated this procedure using commercially available, mass-produced murine embryonic stem cell- and induced pluripotent stem cell-derived myocytes compared with a neonatal mouse ventricular myocyte target phenotype in coupled in vitro assays.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Técnicas de Cultura de Células
/
Miócitos Cardíacos
/
Matriz Extracelular
Limite:
Animals
Idioma:
En
Revista:
Stem Cell Reports
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos