Multiple polyadenylation sites in a large 3'-most exon of the rat insulin-like growth factor II gene.
Biochim Biophys Acta
; 1009(1): 27-34, 1989 Sep 21.
Article
em En
| MEDLINE
| ID: mdl-2477062
ABSTRACT
The rat insulin-like growth factor II (rIGFII) gene produces, in addition to three major mRNA species 3.6 kilobases (kb), 4.6 kb and 3.8 kb in length which represent transcripts from three independent leader-exons, multiple smaller-sized products that distribute broadly in the 1-3 kb region on Northern blots. Structural constituents of these RNAs were analyzed by hybridization with region-specific probes prepared from the entire rIGFII genome. Most of these shorter RNAs contained both 5'-untranslated and coding regions, but only parts of the 3'-untranslated region. At least nine protected sites were mapped within a single 3'-most exon E6 by S1 nuclease analysis. Some but not all of these sites were associated with the upstream polyadenylation signal, AATAAA, or its variants. Since none of the shorter subspecies contained intronic sequences, aberration in splicing is not involved in their generation. Thus, the main parts of submature materials are a collection of discrete species of RNAs, most, if not all, of which are produced by alternative polyadenylation site selection.
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Base de dados:
MEDLINE
Assunto principal:
Poli A
/
RNA
/
Somatomedinas
/
Fator de Crescimento Insulin-Like II
/
Éxons
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Japão