Rapid and parallel quantification of small and large RNA species.
Methods Mol Biol
; 1158: 93-106, 2014.
Article
em En
| MEDLINE
| ID: mdl-24792046
ABSTRACT
Quantitative real-time PCR (qRT-PCR) is a common technique for mRNA quantification. Several methods have been developed in the past few years in order to adapt qRT-PCR also for small non-coding RNAs (sRNA). We here provide a simple and sensitive protocol that allows quantification of mRNAs, selected sRNAs, and long non-coding RNAs (lncRNA) in one cDNA sample by qRT-PCR.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA
/
RNA de Plantas
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Alemanha