Plant-based biopharming of recombinant human lactoferrin.
Cell Biol Int
; 38(9): 989-1002, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-24803187
ABSTRACT
Recombinant proteins are currently recognized as pharmaceuticals, enzymes, food constituents, nutritional additives, antibodies and other valuable products for industry, healthcare, research, and everyday life. Lactoferrin (Lf), one of the promising human milk proteins, occupies the expanding biotechnological food market niche due to its important versatile properties. Lf shows antiviral, antimicrobial, antiprotozoal and antioxidant activities, modulates cell growth rate, binds glycosaminoglycans and lipopolysaccharides, and also inputs into the innate/specific immune responses. Development of highly efficient human recombinant Lf expression systems employing yeasts, filamentous fungi and undoubtedly higher plants as bioreactors for the large-scale Lf production is a biotechnological challenge. This review highlights the advantages and disadvantages of the existing non-animal Lf expression systems from the standpoint of protein yield and its biological activity. Special emphasis is put on the benefits of monocot plant system for Lf expression and the biosafety aspects of the transgenic Lf-expressing plants.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plantas
/
Lactoferrina
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Cell Biol Int
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Ucrânia