Delivery of antiviral small interfering RNA with gold nanoparticles inhibits dengue virus infection in vitro.
J Gen Virol
; 95(Pt 8): 1712-1722, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-24828333
ABSTRACT
Dengue virus (DENV) infection in humans can cause flu-like illness, life-threatening haemorrhagic fever or even death. There is no specific anti-DENV therapeutic or approved vaccine currently available, partially due to the possibility of antibody-dependent enhancement reaction. Small interfering RNAs (siRNAs) that target specific viral genes are considered a promising therapeutic alternative against DENV infection. However, in vivo, siRNAs are vulnerable to degradation by serum nucleases and rapid renal excretion due to their small size and anionic character. To enhance siRNA delivery and stability, we complexed anti-DENV siRNAs with biocompatible gold nanoparticles (AuNPs) and tested them in vitro. We found that cationic AuNP-siRNA complexes could enter Vero cells and significantly reduce DENV serotype 2 (DENV-2) replication and infectious virion release under both pre- and post-infection conditions. In addition, RNase-treated AuNP-siRNA complexes could still inhibit DENV-2 replication, suggesting that AuNPs maintained siRNA stability. Collectively, these results demonstrated that AuNPs were able to efficiently deliver siRNAs and control infection in vitro, indicating a novel anti-DENV strategy.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Antivirais
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Replicação Viral
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Portadores de Fármacos
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Vírus da Dengue
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RNA Interferente Pequeno
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Nanopartículas
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Liberação de Vírus
Limite:
Animals
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos