In-vivo real-time control of protein expression from endogenous and synthetic gene networks.
PLoS Comput Biol
; 10(5): e1003625, 2014 May.
Article
em En
| MEDLINE
| ID: mdl-24831205
ABSTRACT
We describe an innovative experimental and computational approach to control the expression of a protein in a population of yeast cells. We designed a simple control algorithm to automatically regulate the administration of inducer molecules to the cells by comparing the actual protein expression level in the cell population with the desired expression level. We then built an automated platform based on a microfluidic device, a time-lapse microscopy apparatus, and a set of motorized syringes, all controlled by a computer. We tested the platform to force yeast cells to express a desired fixed, or time-varying, amount of a reporter protein over thousands of minutes. The computer automatically switched the type of sugar administered to the cells, its concentration and its duration, according to the control algorithm. Our approach can be used to control expression of any protein, fused to a fluorescent reporter, provided that an external molecule known to (indirectly) affect its promoter activity is available.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Leveduras
/
Proteínas Fúngicas
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Regulação Fúngica da Expressão Gênica
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Engenharia Metabólica
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Modelos Genéticos
Idioma:
En
Revista:
PLoS Comput Biol
Assunto da revista:
BIOLOGIA
/
INFORMATICA MEDICA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Itália