Specific expression of apolipoprotein A-IV in the follicle-associated epithelium of the small intestine.
Dig Dis Sci
; 59(11): 2682-92, 2014 Nov.
Article
em En
| MEDLINE
| ID: mdl-24838500
ABSTRACT
BACKGROUND:
Peyer's patches (PPs), which are covered by specialized follicle-associated epithelium (FAE) including M cells, play a central role in immune induction in the gastrointestinal tract. This study is to investigate a new molecule to characterize PPs.METHODS:
We generated a monoclonal antibody (mAb 10-15-3-3) that specifically reacts to the epithelium of PPs and isolated lymphoid follicles. Target antigen was analyzed by immunoprecipitation and mass spectrometry. Localization and expression of target antigen were evaluated by immunofluorescence, in situ hybridization and real-time PCR.RESULTS:
Immunoprecipitation and mass spectrometry revealed that mAb 10-15-3-3 recognized apolipoprotein A-IV (ApoA-IV), a well-known lipid transporter; this finding was confirmed by the specific reactivity of mAb 10-15-3-3 to cells transfected with the murine ApoA-IV gene. Immunofluorescence using mAb 10-15-3-3 showed intestinal localization of ApoA-IV, in which strong expression of the ApoA-IV protein occurred throughout the entire intestinal epithelium during developing period before weaning but was restricted to the FAE in adult mice. In support of these findings, in situ hybridization showed strong expression of the ApoA-IV gene throughout the entire intestinal epithelium during developing period before weaning, but this expression was restricted to the FAE predominantly and the tips of villi to a lesser extent in adult mice. Deficiency of ApoA-IV had no effect on the organogenesis of PP in mice.CONCLUSIONS:
Our current results reveal ApoA-IV as a novel FAE-specific marker especially in the upper small intestine of adult mice.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Apolipoproteínas A
/
Mucosa Intestinal
/
Intestino Delgado
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Dig Dis Sci
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Japão