Antibodies to PfSEA-1 block parasite egress from RBCs and protect against malaria infection.
Science
; 344(6186): 871-7, 2014 May 23.
Article
em En
| MEDLINE
| ID: mdl-24855263
ABSTRACT
Novel vaccines are urgently needed to reduce the burden of severe malaria. Using a differential whole-proteome screening method, we identified Plasmodium falciparum schizont egress antigen-1 (PfSEA-1), a 244-kilodalton parasite antigen expressed in schizont-infected red blood cells (RBCs). Antibodies to PfSEA-1 decreased parasite replication by arresting schizont rupture, and conditional disruption of PfSEA-1 resulted in a profound parasite replication defect. Vaccination of mice with recombinant Plasmodium berghei PbSEA-1 significantly reduced parasitemia and delayed mortality after lethal challenge with the Plasmodium berghei strain ANKA. Tanzanian children with antibodies to recombinant PfSEA-1A (rPfSEA-1A) did not experience severe malaria, and Kenyan adolescents and adults with antibodies to rPfSEA-1A had significantly lower parasite densities than individuals without these antibodies. By blocking schizont egress, PfSEA-1 may synergize with other vaccines targeting hepatocyte and RBC invasion.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasmodium falciparum
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Anticorpos Antiprotozoários
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Proteínas de Protozoários
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Malária Falciparum
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Vacinas Antimaláricas
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Eritrócitos
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Esquizontes
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Antígenos de Protozoários
Limite:
Adolescent
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Adult
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Animals
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Child
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Humans
País/Região como assunto:
Africa
Idioma:
En
Revista:
Science
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos