Transformation of codeine and codeine-6-glucuronide to opioid analogues by urine adulteration with pyridinium chlorochromate: potential issue for urine drug testing.
Rapid Commun Mass Spectrom
; 28(14): 1609-20, 2014 Jul 30.
Article
em En
| MEDLINE
| ID: mdl-24895258
ABSTRACT
RATIONALE Pyridinium chlorochromate (PCC) is the active ingredient of 'Urine Luck', a commercially available in vitro adulterating agent used to conceal the presence of drugs in a urine specimen. The exposure of codeine and its major glucuronide metabolite codeine-6-glucuronide (C6G) to PCC was investigated to determine whether PCC is an effective masking agent for these opiate compounds. METHODS:
Following the addition of PCC to both spiked and authentic codeine and C6G-positive urine specimens, the samples were monitored using liquid chromatography/mass spectrometry (LC/MS). Stable reaction products were identified and characterized using high-resolution MS analysis and, where possible, nuclear magnetic resonance (NMR) analysis.RESULTS:
It was determined that PCC effectively oxidizes codeine and C6G, thus altering the original codeine-to-C6G ratio in the urine specimen. Four reaction products were identified for codeine codeinone, 14-hydroxycodeinone, 6-O-methylcodeine and 8-hydroxy-7,8-dihydrocodeinone. Similarly, three reaction products were identified for C6G codeinone, codeine and a lactone of C6G (tentative assignment).CONCLUSIONS:
Besides addressing the complications added to interpretation, more investigation is warranted to further determine their potential for use as markers for monitoring the presence of codeine and C6G in urine specimens adulterated with PCC.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Compostos de Piridínio
/
Detecção do Abuso de Substâncias
/
Codeína
Tipo de estudo:
Prognostic_studies
Limite:
Adult
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Rapid Commun Mass Spectrom
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Austrália