Differential expression of p42.3 in low- and high-grade gliomas.
World J Surg Oncol
; 12: 185, 2014 Jun 14.
Article
em En
| MEDLINE
| ID: mdl-24927751
ABSTRACT
BACKGROUND:
Malignant gliomas are the most common form of primary malignant brain tumor. It has recently been suggested that genetic changes are involved in the progression of malignant gliomas. In previous studies, a novel gene, p42.3, was characterized as a tumor-specific gene that encodes a mitosis phase-dependent expression protein which is expressed in gastric cancer, but not in matched normal tissues.METHODS:
In a series of 200 human brain gliomas and 13 normal tissues, we performed RT-PCR and mRNA in situ hybridization for analysis of p42.3 gene expression in gliomas, including astrocytoma (grade 2), oligoastrocytomas (grade 2), anaplastic oligoastrocytomas (grade 3), glioblastomas (grade 4) and normal tissues. Also, the mRNA expression was detected in gliomas by in situ hybridization. After producing polyclonal antibody to p42.3, we further tested p42.3 protein expression in astrocytomas and glioblastomas by immunohistochemistry and Western blot analysis.RESULTS:
Our results demonstrated that overexpression of the p42.3 gene is detected in gliomas, but not in normal brain tissues. Importantly, p42.3 mRNA expression is correlated with the pathological features of gliomas. In addition, p42.3 protein is expressed in both the cytoplasm and the nucleus in astrocytomas, whereas this protein appeared in the cytoplasm in glioblastomas.CONCLUSIONS:
These results indicate that p42.3 might be involved in carcinogenesis as a potential molecular marker for malignant gliomas.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
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Neoplasias Encefálicas
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Biomarcadores Tumorais
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Proteínas de Ciclo Celular
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Glioma
Tipo de estudo:
Observational_studies
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Prognostic_studies
Limite:
Animals
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
World J Surg Oncol
Ano de publicação:
2014
Tipo de documento:
Article