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Acute and chronic B cell depletion disrupts CD4+ and CD8+ T cell homeostasis and expansion during acute viral infection in mice.
Lykken, Jacquelyn M; DiLillo, David J; Weimer, Eric T; Roser-Page, Susanne; Heise, Mark T; Grayson, Jason M; Weitzmann, M Neale; Tedder, Thomas F.
Afiliação
  • Lykken JM; Department of Immunology, Duke University Medical Center, Durham, NC 27710;
  • DiLillo DJ; Department of Immunology, Duke University Medical Center, Durham, NC 27710;
  • Weimer ET; Department of Immunology, Duke University Medical Center, Durham, NC 27710;
  • Roser-Page S; Atlanta Department of Veterans Affairs Medical Center, Decatur, GA 30033;
  • Heise MT; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;
  • Grayson JM; Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, NC 27157; and.
  • Weitzmann MN; Atlanta Department of Veterans Affairs Medical Center, Decatur, GA 30033; Division of Endocrinology, Metabolism and Lipids, Emory University School of Medicine, Atlanta, GA 30322.
  • Tedder TF; Department of Immunology, Duke University Medical Center, Durham, NC 27710; thomas.tedder@duke.edu.
J Immunol ; 193(2): 746-56, 2014 Jul 15.
Article em En | MEDLINE | ID: mdl-24928986
ABSTRACT
B cells provide humoral protection against pathogens and promote cellular immunity through diverse nonclassical effector functions. To assess B cell function in promoting T cell homeostasis, mature B cells were either acutely or chronically depleted in mice using CD20 mAb. Acute B cell depletion in either 2- or 4-mo-old mice significantly reduced spleen and lymph node CD4(+) and CD8(+) T cell numbers, including naive, activated, and Foxp3(+)CD25(+)CD4(+) regulatory T cell subsets. The numbers of IFN-γ- and TNF-α-producing T cells were also significantly reduced. Chronic B cell depletion for 6 mo in aged naive mice resulted in a 40-70% reduction in activated CD4(+) and CD8(+) T cell numbers and 20-50% reductions in IFN-γ-producing T cells. Therefore, B cells were necessary for maintaining naive CD4(+) and CD8(+) T cell homeostasis for subsequent optimal T cell expansion in young and old mice. To determine the significance of this finding, a week of B cell depletion in 4-mo-old mice was followed by acute viral infection with lymphocytic choriomeningitis virus Armstrong. Despite their expansion, activated and cytokine-producing CD4(+) and CD8(+) T cell numbers were still significantly reduced 1 wk later. Moreover, viral peptide-specific CD4(+) and CD8(+) T cell numbers and effector cell development were significantly reduced in mice lacking B cells, whereas lymphocytic choriomeningitis virus titers were dramatically increased. Thus, T cell function is maintained in B cell-depleted mice, but B cells are required for optimal CD4(+) and CD8(+) T cell homeostasis, activation, and effector development in vivo, particularly during responses to acute viral infection.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Linfócitos B / Linfócitos T CD4-Positivos / Linfócitos T CD8-Positivos / Homeostase / Coriomeningite Linfocítica / Vírus da Coriomeningite Linfocítica Limite: Animals Idioma: En Revista: J Immunol Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Linfócitos B / Linfócitos T CD4-Positivos / Linfócitos T CD8-Positivos / Homeostase / Coriomeningite Linfocítica / Vírus da Coriomeningite Linfocítica Limite: Animals Idioma: En Revista: J Immunol Ano de publicação: 2014 Tipo de documento: Article