Actinophytocola gilvus sp. nov., isolated from desert soil crusts, and emended description of the genus Actinophytocola Indananda et al. 2010.
Int J Syst Evol Microbiol
; 64(Pt 9): 3120-3125, 2014 Sep.
Article
em En
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| ID: mdl-24944338
ABSTRACT
An actinomycete strain, designated I12A-02593(T), was isolated from a desert soil crust sample collected in the Shapotou region of Tengger Desert, north-west China. The isolate grew well on International Streptomyces Project (ISP) media 2, 3, 5 and 7, YS and Bennett's agar; it produced spherical bodies and formed clumps on the aerial mycelia on ISP 5 agar plates. Chemotaxonomically, the strain contained meso-diaminopimelic acid as the diagnostic diamino acid, arabinose and galactose as the diagnostic sugars in whole-cell hydrolysates, MK-9(H4) as the sole isoprenoid quinone, and iso-C16ââ0, iso-C16ââ0 2-OH and iso-C16ââ1 H as the major cellular fatty acids, without mycolic acids. The profile of the phospholipids mainly comprised diphosphatidylglycerol, phosphatidylethanolamine and hydroxyphosphatidylethanolamine. The genomic DNA G+C content was 70.1 mol%. The 16S rRNA gene sequence of strain I12A-02593(T) exhibited 96.4-97.4â% similarities with members of the genus Actinophytocola. In the phylogenetic tree based on 16S rRNA gene sequences, the isolate formed a robust cluster with Actinophytocola oryzae NBRC 105245(T), Aactinophytocola timorensis NBRC 105524(T), Actinophytocola corallina NBRC 105525(T), Actinophytocola burenkhanensis NBRC 105883(T)and Actinophytocola xinjiangensis NBRC 106673(T). DNA-DNA hybridization values between strain I12A-02593(T) and the five species of the genus Actinophytocola were all less than 70â%. On the basis of the polyphasic taxonomy evidence, a novel species of the genus Actinophytocola is proposed, with the name Actinophytocola gilvus sp. nov. The type strain is I12A-02593(T) (â=âCPCC 203543(T)â=âDSM 45828(T)â=âNBRC 109453(T)â=âKCTC 29165(T)). An emended description of the genus Actinophytocola is also provided.
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Microbiologia do Solo
/
Actinomycetales
País/Região como assunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2014
Tipo de documento:
Article