Prostaglandin synthesis by isolated cells of rat uterus: production rates, and effects of indomethacin and histamine.
Prostaglandins Leukot Essent Fatty Acids
; 35(1): 51-6, 1989 Jan.
Article
em En
| MEDLINE
| ID: mdl-2494675
ABSTRACT
Luminal epithelial and residual cells (mainly of the endometrial stromal tissue) of proestrous rat uteri have been isolated and cultured in defined medium. The prostaglandins produced during a short-term incubation (2 h) in the presence of 10 microM arachidonic acid (to optimize PG production) were determined by direct assay of the culture medium. For the epithelial cells, PGF2 alpha was produced in greatest amounts, followed by 6-keto PGF1 alpha and PGE, while low levels were synthesized by the residual cells. The synthesis of PGF2 alpha by the epithelial cells was inhibited by incorporating indomethacin into the medium and an IC50 value of 2.3 microM was obtained. Incubations performed with histamine in the absence of exogenous arachidonic acid indicated that the pathways for the production of individual prostaglandins were followed to different relative extents, with the production of 6-keto PGF1 alpha being enhanced for both groups of cells when compared to incubations with arachidonic acid.
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Base de dados:
MEDLINE
Assunto principal:
Útero
/
Prostaglandinas
Limite:
Animals
Idioma:
En
Revista:
Prostaglandins Leukot Essent Fatty Acids
Assunto da revista:
ENDOCRINOLOGIA
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Nova Zelândia