Stability studies of binding and functional anti-vaccine antibodies.
Bioanalysis
; 6(10): 1385-93, 2014 May.
Article
em En
| MEDLINE
| ID: mdl-24958122
ABSTRACT
BACKGROUND:
As most vaccines exert their protective capacity by eliciting pathogen-specific antibodies, antibody assays assessing immunogenicity of vaccines in development should be well characterized. Part of the validation of immunogenicity assays for vaccines is the study of stability of antibodies in serum. Materials &methods:
Stability of antibodies in human serum was assessed by circumsporozoite-binding IgG ELISA designed for assessing the immunogenicity of a malaria vaccine under development, adenovirus neutralization assay, designed to assess neutralizing antibodies against adenovirus and commercially available test kits for hepatitis A and B.RESULTS:
Stability studies indicated stability of serum-binding IgG antibodies and serum-neutralizing antibodies in long-term storage below -65°C and -20°C; short-term storage; multiple freeze/thaw rounds; during shipment; and during heat inactivation.CONCLUSION:
RESULTS have shown the stability of both binding and functional polyclonal antibodies in human serum under stable storage and common usage circumstances.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Vacinas
/
Anticorpos Neutralizantes
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2014
Tipo de documento:
Article