Modular riboswitch toolsets for synthetic genetic control in diverse bacterial species.
J Am Chem Soc
; 136(30): 10615-24, 2014 Jul 30.
Article
em En
| MEDLINE
| ID: mdl-24971878
ABSTRACT
Ligand-dependent control of gene expression is essential for gene functional analysis, target validation, protein production, and metabolic engineering. However, the expression tools currently available are difficult to transfer between species and exhibit limited mechanistic diversity. Here we demonstrate how the modular architecture of purine riboswitches can be exploited to develop orthogonal and chimeric switches that are transferable across diverse bacterial species, modulating either transcription or translation, to provide tunable activation or repression of target gene expression, in response to synthetic non-natural effector molecules. Our novel riboswitch-ligand pairings are shown to regulate physiologically important genes required for bacterial motility in Escherichia coli and cell morphology in Bacillus subtilis. These findings are relevant for future gene function studies and antimicrobial target validation, while providing new modular and orthogonal regulatory components for deployment in synthetic biology regimes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
/
Regulação Bacteriana da Expressão Gênica
/
Escherichia coli
/
Riboswitch
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Am Chem Soc
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Reino Unido