Ratiometric imaging of pH probes.
Methods Cell Biol
; 123: 429-48, 2014.
Article
em En
| MEDLINE
| ID: mdl-24974041
ABSTRACT
Measurement of intracellular pH can be readily accomplished using tools and methods described in this chapter. We present a discussion of technical considerations of various ratiometric pH-sensitive probes including dyes and genetically encoded sensors. These probes can be used to measure pH across physical scales from macroscopic whole-mount tissues down to organelles and subcellular domains. We describe protocols for loading pH-sensitive probes into single cells or tissues and discuss ratiometric image acquisition and analysis.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Fluoresceínas
/
Corantes Fluorescentes
Limite:
Animals
Idioma:
En
Revista:
Methods Cell Biol
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos