Engineering terpene biosynthesis in Streptomyces for production of the advanced biofuel precursor bisabolene.
ACS Synth Biol
; 4(4): 393-9, 2015 Apr 17.
Article
em En
| MEDLINE
| ID: mdl-25006988
ABSTRACT
The past decade has witnessed a large influx of research toward the creation of sustainable, biologically derived fuels. While significant effort has been exerted to improve production capacity in common hosts, such as Escherichia coli or Saccharomyces cerevisiae, studies concerning alternate microbes comparatively lag. In an effort to expand the breadth of characterized hosts for fuel production, we map the terpene biosynthetic pathway in a model actinobacterium, Streptomyces venezuelae, and further alter secondary metabolism to afford the advanced biofuel precursor bisabolene. Leveraging information gained from study of the native isoprenoid pathway, we were able to increase bisabolene titer nearly 5-fold over the base production strain, more than 2 orders of magnitude greater than the combined terpene yield in the wild-type host. We also explored production on carbon sources of varying complexity to, notably, define this host as one able to perform consolidated bioprocessing.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sesquiterpenos
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Streptomyces
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Biocombustíveis
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Engenharia Metabólica
Idioma:
En
Revista:
ACS Synth Biol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos