Epigenetic modification agents improve genomic methylation reprogramming in porcine cloned embryos.
J Reprod Dev
; 60(5): 377-82, 2014.
Article
em En
| MEDLINE
| ID: mdl-25047549
ABSTRACT
Incomplete DNA methylation reprogramming in cloned embryos leads to low cloning efficiency. Our previous studies showed that the epigenetic modification agents 5-aza-2'-deoxycytidine (5-aza-dC) or trichostatin A (TSA) could enhance the developmental competence of porcine cloned embryos. Here, we investigated genomic methylation dynamics and specific gene expression levels during early embryonic development in pigs. In this study, our results showed that there was a typical wave of DNA demethylation and remethylation of centromeric satellite repeat (CenRep) in fertilized embryos, whereas in cloned embryos, delayed demethylation and a lack of remethylation were observed. When cloned embryos were treated with 5-aza-dC or TSA, CenRep methylation reprogramming was improved, and this was similar to that detected in fertilized counterparts. Furthermore, we found that the epigenetic modification agents, especially TSA, effectively promoted silencing of tissue specific genes and transcription of early embryo development-related genes in porcine cloned embryos. In conclusion, our results showed that the epigenetic modification agent 5-aza-dC or TSA could improve genomic methylation reprogramming in porcine cloned embryos and regulate the appropriate expression levels of genes related to early embryonic development, thereby resulting in high developmental competence.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Suínos
/
Metilação de DNA
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Clonagem de Organismos
/
Epigênese Genética
Limite:
Animals
Idioma:
En
Revista:
J Reprod Dev
Assunto da revista:
MEDICINA REPRODUTIVA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China