Structure, stability, and IgE binding of the peach allergen Peamaclein (Pru p 7).
Biopolymers
; 102(5): 416-25, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-25130872
ABSTRACT
Knowledge of the structural properties of allergenic proteins is a necessary prerequisite to better understand the molecular bases of their action, and also to design targeted structural/functional modifications. Peamaclein is a recently identified 7 kDa peach allergen that has been associated with severe allergic reactions in sensitive subjects. This protein represents the first component of a new allergen family, which has no 3D structure available yet. Here, we report the first experimental data on the 3D-structure of Peamaclein. Almost 75% of the backbone resonances, including two helical stretches in the N-terminal region, and four out of six cysteine pairs have been assigned by 2D-NMR using a natural protein sample. Simulated gastrointestinal digestion experiments have highlighted that Peamaclein is even more resistant to digestion than the peach major allergen Pru p 3. Only the heat-denatured protein becomes sensitive to intestinal proteases. Similar to Pru p 3, Peamaclein keeps its native 3D-structure up to 90°C, but it becomes unfolded at temperatures of 100-120°C. Heat denaturation affects the immunological properties of both peach allergens, which lose at least partially their IgE-binding epitopes. In conclusion, the data collected in this study provide a first set of information on the molecular properties of Peamaclein. Future studies could lead to the possible use of the denatured form of this protein as a vaccine, and of the inclusion of cooked peach in the diet of subjects allergic to Peamaclein.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Imunoglobulina E
/
Alérgenos
Limite:
Humans
Idioma:
En
Revista:
Biopolymers
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Itália