Spherical nucleic acids as a divergent platform for synthesizing RNA-nanoparticle conjugates through enzymatic ligation.
ACS Nano
; 8(9): 8837-43, 2014 Sep 23.
Article
em En
| MEDLINE
| ID: mdl-25144723
ABSTRACT
Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA-nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Ligases
/
RNA
/
Nanotecnologia
/
Nanopartículas
/
Conformação de Ácido Nucleico
Limite:
Humans
Idioma:
En
Revista:
ACS Nano
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos