[Identification of Bupleurum chinense and B. longiradiatum based on ITS2 barcode].
Zhongguo Zhong Yao Za Zhi
; 39(12): 2160-3, 2014 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-25244736
ABSTRACT
In this study, ITS2 barcode was used to identify Bupleurum chinense and B. longiradiatum. The ITS2 regions of 48 samples were amplified and sequenced. The sequences obtained above were aligned and the K2P distances were calculated. We used three methods, BLAST1, nearest distance and phylogenetic tree (NJ-tree), to test the identification ability. The results showed that the maximum intraspecific genetic distance of B. chinense was 0.013, and the minimum interspecific genetic distance between B. chinense and B. longiradiatum was 0.049. The NJ-tree can easily identify B. chinense and B. longiradiatum. Therefore, the ITS2 barcode is suitable to identify B. chinense and B. longiradiatum.
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Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
/
DNA de Plantas
/
DNA Espaçador Ribossômico
/
Bupleurum
/
Código de Barras de DNA Taxonômico
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Assunto da revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Ano de publicação:
2014
Tipo de documento:
Article