DNA-hosted Hoechst dyes: application for label-free fluorescent monitoring of endonuclease activity and inhibition.

Jiang, Xiao-Qin; Guo, Su-Miao; Zhang, Min; Zhou, Ming; Ye, Bang-Ce

Jiang, Xiao-Qin; Guo, Su-Miao; Zhang, Min; Zhou, Ming; Ye, Bang-Ce.

Afiliação

Jiang XQ; Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China. bcye@ecust.edu.cn.

Analyst ; 139(22): 5682-5, 2014 Nov 21.

Article em En | MEDLINE | ID: mdl-25272021

ABSTRACT

ABSTRACT

A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.

Assuntos

DNA/química; Endonucleases/metabolismo; Corantes Fluorescentes/química; Endonucleases/antagonistas & inibidores

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Endonucleases / Corantes Fluorescentes Idioma: En Revista: Analyst Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China

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