Performance assessment of the BD MAX Cdiff assay in comparison to Xpert C. difficile assay in a setting with very low prevalence of toxigenic Clostridium difficile PCR ribotype 027.

Chiang, Donald; Ng, Sally; La, My-Van; Jureen, Roland; Lin, Raymond T P; Teo, Jeanette W P

Performance assessment of the BD MAX Cdiff assay in comparison to Xpert C. difficile assay in a setting with very low prevalence of toxigenic Clostridium difficile PCR ribotype 027.

Chiang, Donald; Ng, Sally; La, My-Van; Jureen, Roland; Lin, Raymond T P; Teo, Jeanette W P.

Afiliação

Chiang D; Department of Laboratory Medicine, Microbiology Unit, National University Hospital, Singapore 119074, Singapore.
Ng S; Department of Laboratory Medicine, Microbiology Unit, National University Hospital, Singapore 119074, Singapore.
La MV; National Public Health Laboratory, Ministry of Health, Singapore.
Jureen R; Department of Laboratory Medicine, Microbiology Unit, National University Hospital, Singapore 119074, Singapore.
Lin RT; Department of Laboratory Medicine, Microbiology Unit, National University Hospital, Singapore 119074, Singapore.
Teo JW; Department of Laboratory Medicine, Microbiology Unit, National University Hospital, Singapore 119074, Singapore. Electronic address: Jeanette_Teo@nuhs.edu.sg.

Anaerobe ; 30: 156-8, 2014 Dec.

Article em En | MEDLINE | ID: mdl-25305151

RESUMO

In a clinical setting with low prevalence of 'epidemic' PCR ribotype 027, the BD MAX Cdiff assay was found to be a suitable alternative to the Xpert C. difficile assay for the detection of toxigenic Clostridium difficile in samples which are reflex PCR tested after obtaining a discrepant immunoassay result. There was no significant difference between the sensitivities and specificities of both commercial molecular assays.

Assuntos

Técnicas Bacteriológicas/métodos; Clostridioides difficile/isolamento & purificação; Infecções por Clostridium/diagnóstico; Infecções por Clostridium/microbiologia; Técnicas de Diagnóstico Molecular/métodos; Clostridioides difficile/classificação; Clostridioides difficile/genética; Infecções por Clostridium/epidemiologia; Humanos; Reação em Cadeia da Polimerase; Prevalência; Ribotipagem; Sensibilidade e Especificidade

Palavras-chave

Clostridium; Nosocomial infection; Real-time PCR

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Clostridioides difficile / Técnicas Bacteriológicas / Infecções por Clostridium / Técnicas de Diagnóstico Molecular Tipo de estudo: Diagnostic_studies / Evaluation_studies / Prevalence_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Anaerobe Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Singapura

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