Discrimination between random and non-random processes in early bacterial colonization on biomaterial surfaces: application of point pattern analysis.
Biofouling
; 30(9): 1023-33, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-25329612
ABSTRACT
The dynamics of adhesion and growth of bacterial cells on biomaterial surfaces play an important role in the formation of biofilms. The surface properties of biomaterials have a major impact on cell adhesion processes, eg the random/non-cooperative adhesion of bacteria. In the present study, the spatial arrangement of Escherichia coli on different biomaterials is investigated in a time series during the first hours after exposure. The micrographs are analyzed via an image processing routine and the resulting point patterns are evaluated using second order statistics. Two main adhesion mechanisms can be identified random adhesion and non-random processes. Comparison with an appropriate null-model quantifies the transition between the two processes with statistical significance. The fastest transition to non-random processes was found to occur after adhesion on PTFE for 2-3 h. Additionally, determination of cell and cluster parameters via image processing gives insight into surface influenced differences in bacterial micro-colony formation.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Materiais Biocompatíveis
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Biofilmes
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Escherichia coli
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Incrustação Biológica
Tipo de estudo:
Clinical_trials
/
Prognostic_studies
Idioma:
En
Revista:
Biofouling
Assunto da revista:
BIOLOGIA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Alemanha