In-planta sporulation phenotype: a major life history trait to understand the evolution of Alnus-infective Frankia strains.
Environ Microbiol
; 17(9): 3125-38, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-25335453
ABSTRACT
Two major types of Frankia strains are usually recognized, based on the ability to sporulate in-planta spore-positive (Sp+) and spore-negative (Sp-). We carried out a study of Sp+ and Sp- Frankia strains based on nodules collected on Alnus glutinosa, Alnus incana and Alnus viridis. The nodules were phenotyped using improved histology methods, and endophytic Frankia strain genotype was determined using a multilocus sequence analysis approach. An additional sampling was done to assess the relation between Sp+ phenotype frequency and genetic diversity of Frankia strains at the alder stand scale. Our results revealed that (i) Sp+ and Sp- Alnus-infective Frankia strains are genetically different even when sampled from the same alder stand and the same host-plant species; (ii) there are at least two distinct phylogenetic lineages of Sp+ Frankia that cluster according to the host-plant species and without regard of geographic distance and (iii) genetic diversity of Sp+ strains is very low at the alder stand scale compared with Sp- strains. Difference in evolutionary history and genetic diversity between Sp+ and Sp- Frankia allows us to discuss the possible ecological role of in-planta sporulation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Esporos Bacterianos
/
Alnus
/
Frankia
Idioma:
En
Revista:
Environ Microbiol
Assunto da revista:
MICROBIOLOGIA
/
SAUDE AMBIENTAL
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
França