Clonal culture of adult mouse lung epithelial stem/progenitor cells.
Methods Mol Biol
; 1235: 231-41, 2015.
Article
em En
| MEDLINE
| ID: mdl-25388397
ABSTRACT
Clonal culture of stem cells is crucial for their identification, and the characterization of the cellular and molecular mechanisms that regulate their proliferation and differentiation. In the adult mouse lung, epithelial stem/progenitor cells are defined by the phenotype CD45(neg) CD31(neg) EpCAM(pos) CD104(pos) CD24(low). Here we describe a tissue dissociation and flow cytometry strategy for the detection and isolation of adult mouse lung epithelial stem/progenitor cells, and a three-dimensional colony-forming assay for their clonal culture in vitro.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Separação Celular
/
Células Epiteliais
/
Células-Tronco Adultas
/
Citometria de Fluxo
/
Pulmão
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2015
Tipo de documento:
Article