4Pi microscopy of the nuclear pore complex.
Methods Mol Biol
; 1251: 193-211, 2015.
Article
em En
| MEDLINE
| ID: mdl-25391801
ABSTRACT
4Pi microscopy is a far-field fluorescence microscopy technique, in which the wave fronts of two opposing illuminating beams are adjusted to constructively interfere in a common focus. This yields a diffraction pattern in the direction of the optical axis, which essentially consists of a main focal spot accompanied by two smaller side lobes. At optimal conditions, the main peak of this so-called point spread function has a full width at half maximum fixed phrase of 100 nm in the direction of the optical axis, and thus is 6-7-fold smaller than that of a confocal microscope. In this chapter, we describe the basic features of 4Pi microscopy and its application to cell biology using the example of the nuclear pore complex, a large protein assembly spanning the nuclear envelope.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Citológicas
/
Poro Nuclear
/
Microscopia de Fluorescência
/
Microscopia de Interferência
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos