Evaluation of the combination of a dispersive liquid-liquid microextraction method with micellar electrokinetic chromatography coupled to mass spectrometry for the determination of estrogenic compounds in milk and yogurt.
Electrophoresis
; 36(4): 615-25, 2015 Feb.
Article
em En
| MEDLINE
| ID: mdl-25394185
ABSTRACT
In this work, the suitability of a methodology based on dispersive liquid-liquid microextraction (DLLME) has been evaluated for the extraction of four endoestrogens (estriol, 17α-estradiol, 17ß-estradiol, and estrone), an exoestrogen (17α-etynylestradiol), and a mycotoxin (zearalenone), together with some of their major metabolites (2-methoxyestradiol, α-zearalanol, ß-zearalanol, α-zearalenol, and ß-zearalenol) from different types of milk (whole and skimmed cow milk and semiskimmed goat milk) and whole natural yogurt. The methodology includes a previous protein precipitation with acidified ACN and a defatting step with n-hexane. Separation of the analytes, determination, and quantification were developed by MEKC coupled to ESI-MS using a BGE containing an aqueous solution of ammonium perfluorooctanoate as MS friendly surfactant. Calibration, precision, and accuracy studies of the described DLLME-MEKC-MS/MS method were evaluated obtaining a good linearity and LODs in the low micrograms per liter range.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Iogurte
/
Cromatografia Capilar Eletrocinética Micelar
/
Leite
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Estrogênios
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Microextração em Fase Líquida
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Itália