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Role of 5-hydroxymethylcytosine level in diagnosis and prognosis prediction of intrahepatic cholangiocarcinoma.
Dong, Zhao-Ru; Zhang, Chi; Cai, Jia-Bin; Zhang, Peng-Fei; Shi, Guo-Ming; Gao, Dong-Mei; Sun, Hui-Chuan; Qiu, Shuang-Jian; Zhou, Jian; Ke, Ai-Wu; Fan, Jia.
Afiliação
  • Dong ZR; Liver Cancer Institute and Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, People's Republic of China.
Tumour Biol ; 36(4): 2763-71, 2015 Apr.
Article em En | MEDLINE | ID: mdl-25480415
ABSTRACT
The loss of 5-hydroxymethylcytosine (5-hmC) has been identified as an epigenetic hallmark in several malignancies. However, its role in intrahepatic cholangiocarcinoma (ICC) is still unknown. Our study aims to investigate the level of 5-hmC in diagnosis and prognosis prediction of ICC. The 5-hmC levels were detected using dot blot, tissue microarray technique and immunohistochemical method, and the correlation between 5-hmC level and ICC clinicopathological parameters was analysed. Compared with matched liver tissues, most of ICC tissues presented with the loss of 5-hmC. Furthermore, the subgroups of cirrhotic and poor differentiation tissues showed the lowest level of 5-hmC. We found that 5-hmC level in non-elevated ICC patients was significantly related to lymph node metastasis and TNM stage and not related to vessel invasion, sex, age, HBV, cirrhosis or degree of differentiation. ICC patients with high TNM stage (stages III and IV) and lymph node metastases had significantly lower 5-hmC level than those with low TNM stage (stages I and II) and no lymph node metastases. Further analysis showed that low 5-hmC level is significantly correlated with worse overall survival (OS) and disease-free survival (DFS). Importantly, multivariate analysis indicated that 5-hmC level, tumour diameter, lymphatic metastasis and tumour differentiation could be used as independent prognostic factors for ICC. The loss of 5-hmC is implicated in the progression of ICC. Our results can contribute to the diagnostic ability and postoperative surveillance of ICC patients.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Prognóstico / Neoplasias dos Ductos Biliares / Colangiocarcinoma / Citosina Tipo de estudo: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Limite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Tumour Biol Assunto da revista: NEOPLASIAS Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Prognóstico / Neoplasias dos Ductos Biliares / Colangiocarcinoma / Citosina Tipo de estudo: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Limite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Tumour Biol Assunto da revista: NEOPLASIAS Ano de publicação: 2015 Tipo de documento: Article